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. Author manuscript; available in PMC: 2020 Oct 22.
Published in final edited form as: J Control Release. 2020 Apr 21;323:269–281. doi: 10.1016/j.jconrel.2020.04.030

Fig. 2.

Fig. 2

Nanobody-PS conjugates bind to cell lines according to their HER2 expression levels and upon illumination induce cytotoxicity to cells with high HER2 expression. A. Dilution series of 1D5-PS and 1D5-18A12-PS were incubated with cells for 1 h 30 min at 4 °C to determine apparent affinity. Bound nanobodies are directly detected through fluorescence of the PS. B. Total fluorescence intensity associated with cells after 30 min incubation at 37 °C with a concentration range of 1D5-PS or 1D5-18A12-PS. C. Cell viability determined 24 h after illumination with 10 J∙cm−2. PDT with HER2 overexpressing cells and low HER2 expressing cells and 1D5-PS or 1D5-18A12-PS.