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. Author manuscript; available in PMC: 2020 Nov 3.
Published in final edited form as: Nature. 2020 Mar 4;579(7800):603–608. doi: 10.1038/s41586-020-2059-5

Fig. 3. AA-ICL repair is mediated by REV1.

Fig. 3

(a) Scheme of products detected on the sequencing gel in (c). (b) REV1 Western blot for mock and REV1-depleted extract compared to a titration of undepleted extract. Nine independent experiments. (c) Plasmids were replicated in mock or REV1-depleted (ΔREV1) extracts, repair intermediates were digested and separated on a sequencing gel alongside a sequencing ladder. White arrows: 0 products, dark grey arrows: –1 products, light grey arrows: 0/-1 products. Three independent experiments. (d) Scheme of the primer extension assay. Late repair products are digested with AflIII and BamHI and used as template for primer extension from labelled TOP or BOT (bottom) primers. (e) Primer extension products were separated by denaturing PAGE. Four independent experiments.