Skip to main content
. Author manuscript; available in PMC: 2020 Nov 3.
Published in final edited form as: Nature. 2020 Mar 4;579(7800):603–608. doi: 10.1038/s41586-020-2059-5

Extended Data Figure 3. Reduced acetaldehyde ICLs are repaired by the Fanconi Pathway.

Extended Data Figure 3

(a) MALDI data confirms identity and stability of the reduced ICL. Three independent experiments. (b) The indicated plasmids were replicated in Xenopus egg extracts in presence or absence of p97i and the repair intermediates were analysed using the NotI digestion assay. Increase in intensity of the 44 nt band indicates ongoing replication and repair. A band with higher mobility, probably generated from end joining activity in some extracts is indicated (*). Quantification of repair based on this gel is shown in Main Figure 1j. Three independent experiments. (c) Independent experimental duplicate of main figure 1j. (d) Independent experimental triplicate of main figure 1j.