(A) FAM-YVAD-FMK provides localisation of YVADase activity in Papaver pollen tubes. (B) MT-CMXRos mitochondrial signal. (C) MT-CMXRos and FAM-YVAD-FMK merge shows considerable co-localisation. Detail of (D) FAM-YVAD-FMK, (E) MT-CMXRos and (F) co-localisation. Scale bar, 10 µm. Reproduced from Bosch M, Poulter NS, Perry RM, Wilkins KA, Franklin-Tong VE. 2010. Characterization of a legumain/vacuolar processing enzyme and YVADase activity in Papaver pollen. Plant Molecular Biology 74, 381-393, by kind permission of Springer Nature (www.springernature.com).