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. Author manuscript; available in PMC: 2020 Nov 4.
Published in final edited form as: Nat Struct Mol Biol. 2020 May 4;27(5):500–510. doi: 10.1038/s41594-020-0406-8

Extended Data Fig. 6. RNA promotes CDK11 recruitment to the FLASH-containing RDH chromatin.

Extended Data Fig. 6

a, Workflow of nuclear pellet fractionation procedure.

b,c, Western blot analysis of: immunoprecipitates of endogenous FLASH from cell lysates either treated or not with RNase A (b), association of indicated factors in soluble and insoluble fractions of chromatin either treated or not with RNase A/T1 (c) Antibodies are shown on the side of the panels.

d, Western blot analysis of immunoprecipitates of endogenous FLASH from HCT116 cells carrying stably integrated flag-tagged CDK11 (F-CDK11), its indicated deletion mutants and control flag-tagged empty vector (F-EV) (right panel). Western blot analyses of inputs into FLASH immunoprecipitations are shown (left panel). Amounts of flag-tagged CDK11 proteins were monitored by anti-flag antibody.

e, Western blot analysis of endogenous (endo) and flag-tagged CDK11 protein levels in the cells treated with amanitin or triptolide or untreated control (CTRL) used for F-CDK11 ChIP-qPCR (Fig. 4e). Antibodies are shown on the side of the panels.