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. Author manuscript; available in PMC: 2020 Nov 4.
Published in final edited form as: Nat Struct Mol Biol. 2020 May 4;27(5):500–510. doi: 10.1038/s41594-020-0406-8

Figure 4. RNA promotes CDK11 recruitment to the RDH chromatin.

Figure 4

a, Schematic diagram highlighting the kinase domain and basic region of human CDK11 protein.

b, Metagene analyses of F-CDK11 and F-CDK11 (226-783) iCLIP binding at all RDH transcripts from the TSS to the SL. iCLIP data k-means clustered, based on RNA-seq expression (high, medium and low). n=4 biologically independent experiments.

c, Biodalliance genome browser view of F-CDK11, F-CDK11 (226-783) and uncrosslinked control (no UV) iCLIP binding at HIST1H3B transcript. Stem loop (SL) is indicated by a black line.

d, Western blot analysis of association of the indicated factors in soluble and insoluble fractions of chromatin either treated or not treated with RNase A/T1. Arrows mark phosphorylated (upper) and non-phosphorylated (lower) forms of RNAPII. For CDK11, long and short exposures of the film are shown.

e, CDK11 ChIP-qPCR on RDH genes in HCT116 cells expressing stably integrated F-CDK11 and treated either with Amanitin (4 μg/ml) or Triptolide (10 μM) or untreated (CTRL). n=4 biologically independent experiments, error bars=SEM, *P<0.05, Student´s t-test, Ir=intergenic region. Source data for panel e are available with the paper on line.