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. Author manuscript; available in PMC: 2020 Nov 4.
Published in final edited form as: Nat Struct Mol Biol. 2020 May 4;27(5):500–510. doi: 10.1038/s41594-020-0406-8

Extended Data Fig. 2. CDK11 and FLASH interact directly and are present on the chromatin of RDH genes.

Extended Data Fig. 2

a, b, Western blot analyses of immunoprecipitates of endogenous CDK11 (a) and flag-tagged FLASH (F-FLASH) (b) from HCT116 cells expressing F-FLASH. The blots were probed against proteins indicated on the side.

c, Correlation analyses of CDK11 and FLASH co-occupancy genome-wide (10 kb bins) (top panel) and on RDH genes (bottom panel). Spearman correlation coefficient between indicated ChIP-seq samples is shown and correlation strength is indicated by colour code. FLASH ChIP-seq from hTERT and U2OS cells.

d, Western blot analyses of FLASH and CDK11 protein levels in cell lysates used for the CDK11 and FLASH ChIP-qPCR experiments in Fig. 2e and Extended Data Fig. 2e, respectively. FUS is a loading control. Representative replicate is shown.

e, Endogenous FLASH ChIP-qPCR on indicated RDH genes or control intergenic region (Ir) in HCT116 cells treated either with control (CTRL) or FLASH siRNAs for 24 h. n=3 biologically independent experiments, error bars=SEM, *P<0.05, Student´s two-sided t-test.