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. Author manuscript; available in PMC: 2021 Mar 4.
Published in final edited form as: Science. 2020 Sep 4;369(6508):1220–1227. doi: 10.1126/science.aba4904

Fig. 5. Blind spot for start-site recognition.

Fig. 5

RelE cleavage assay (materials and methods) shows a blind spot for start-site selection for mRNAs with a start codon less than 30 to 40 nucleotides from the 5′ end. All five mRNAs tested have an AUG at position 50 just beyond the blind spot and a second AUG at positions downstream (60 nucleotides) or upstream (40, 30, 19, and 10 nucleotides) of it. The main site of cleavage is at AUG 50 in all cases, showing that initiation occurs primarily at the first start codon downstream of the blind spot, and there is little or no cleavage at start codons for 10 to 40 nucleotides.