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. Author manuscript; available in PMC: 2020 Nov 13.
Published in final edited form as: Nat Plants. 2018 May 28;4(6):365–375. doi: 10.1038/s41477-018-0160-7

Figure 4. ORE1 and KIR1 redundantly control stigma lifespan.

Figure 4

a) Representative snapshots of the webcam analysis of Col-0 and diverse ore1 and kir1 mutants. b) Quantitative analysis of the webcam experiments. In order to integrate several independent experiments in a comparative analysis, the lifespan of the stigmata in mutant lines was normalized to the Col-0 control in the individual experiments. While ore1 mutant stigmata live slightly but not significantly longer, kir1 and kir1 ore1 mutant stigmata are significantly longer living. Different independent lines of ORE1-SRDX and KIR1-SRDX show even stronger stigma life-extending effects. Black box-and-whiskers show Col-0 controls (from left to right, mean±SEM=1±0.014, 1±0.014, 1±0.014, 1±0.015, 1±0.017, 1±0.018, 1±0.018, N=15,15,15,20,13,20,17), red box-and-whiskers show mutants (from left to right, mean±SEM=1.101±0.020, 1.557±0.099, 2.139±0.091, 2.402±0.127, 2.472±0.213, 2.789±0.135, 3.881±0.196, N=15,15,15,19,14,20,14). Statistical differences were calculated using two-way ANOVA with multiple comparisons by Fisher’s LSD test. Black asterisks indicate the comparison between Col-0 and mutants, red asterisks show the comparison between different mutants, significance levels at * P<0.05.