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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Nat Microbiol. 2020 Oct 26;5(12):1588–1597. doi: 10.1038/s41564-020-00801-y

Extended Data Figure 8. Salmonella expressing flagellin from non-pathogenic E. coli (M525P fliC MG1655) is motile but impaired at activating the NLRC4 inflammasome in macrophages.

Extended Data Figure 8

Comparison of the motility diameter between S. Typhimurium M525P, M525P fliC MG1655 and SL1344. Data are expressed as % of the diameter achieved by the SL1344. b-d, Unprimed bone marrow derived macrophages from wild-type and nlrc4-/- mice were infected for lh, 2h, 6h and 24h with S. Typhimurium M525P or M525P fliCMG1655 in logarithmic growth. b, Cytotoxicity was determined by measuring levels of LDH released in the cell culture supernatant at an MOI of 50. c, Levels of IL-lβ and d, TNF-α were determined by ELISA in the cell culture supernatant at an MOI of 1. Data has been pooled from 6 independent experiments and shown as mean ± SEM in a. Data are representative of two independent experiments in b-d and shown as mean of triplicate wells ± SEM.