. Author manuscript; available in PMC: 2021 May 1.

Published in final edited form as: Nature. 2020 Sep 9;587(7834):495–498. doi: 10.1038/s41586-020-2696-8

Extended Data Table 1. Data collection and processing parameters for M1 within virions and VLPs.

	Influenza A/HK68 virus	Influenza A/HK68 HANAM1M2 VLP
*Data collection and processing*
Magnification	81,000 X	81,000 X
Voltage (keV)	300	300
Detector	Gatan Quantum K2	Gatan Quantum K2
Energy-filter	Yes	Yes
Slit width (eV)	20	20
Pixel size(Å)	1.70	1.78
Defocus range (μm)	1.5 to 4.5	2 to 6
Defocus step (μm)	0.25	0.25
Tilt range (min/max, step)	-60/60°, 3°	-60/60°, 3°
Tilt scheme	Dose-symmetrical (Hagen scheme)	Dose-symmetrical (Hagen scheme)
Total Dose (electrons/Å²)	-130-150	-120
Frame number	20	20
Tomograms used for Ml/acquired	53/105	20/60

Tomograms/viruses used for final structure	5	4
Final subtomograms set A NTD/CTD	6025/5934	6936/7164
Final subtomograms set B NTD/CTD	6149/6147	7611/7822
Symmetry imposed	none	none
B-factor	-620	-665
Map resolution isotropy (Å) at 0.143 FSC threshold	5.8-15	6.8-9.8
Global resolution (Å) at 0.143 FSC threshold	7.6	8.2