Extended Data Table 2. Data collection and processing parameters for in vitro helical assembly of M1.
| Ml helical assembly (EMDB-11079) (PDB 6Z5L) | |
|---|---|
| Data collection and processing | |
| Magnification | 105000 |
| Voltage (kV) | 300 |
| Electron exposure (e-/Å2) | 47.1 |
| Defocus range (μm) | 1 to 3 |
| Pixel size(Å) | 1.128 |
| Symmetry imposed | Dl helical |
| Initial particle images (no.) | 463152 |
| Final particle images (no.) | 17984 |
| Map resolution (Å; FSC=0.143) | 3.81 |
| Helical rise (Å) | 3.08 |
| Helical twist (°) | 11.11 |
| Refinement | |
| Initial model used (PDB code) | 1AA7 |
| Model resolution (Å) (FSC=0.5) | 3.90 |
| Map sharpening B factor (Å2) | -115 |
| Model composition | |
| Non-hydrogen atoms | 1937 |
| Protein residues | 251 |
| B factors (Å2) | |
| Protein | 96.4 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.005 |
| Bond angles (°) | 1.010 |
| Validation | |
| MolProbity score | 1.30 |
| Clashscore | 1.79 |
| Poor rotamers (%) | 0 |
| Ramachandran plot | |
| Favored (%) | 94.78 |
| Allowed (%) | 5.52 |
| Disallowed (%) | 0 |