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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Nat Metab. 2020 Nov 9;2(11):1350–1367. doi: 10.1038/s42255-020-00306-2

Figure 8. Pharmacological inhibition of c-Rel limits fibrogenesis in human precision cut liver slices.

Figure 8

(a-b) Representative images and histological quantification of (a) Picrosirius red (p value = 0.0009) and (b) αSMA (p value = 0.0006) stained liver slices ± TGFβ1 ± IT-603 therapy. Red line denotes the value for the T=0 slice. (c-d) Quantification of (c) soluble collagen (p value = 0.0023) and (d) the neo-epitope pro C3 (p value 0.0286) released from fibrotic liver slices ± IT-603 therapy. (e) Graph showing average LDH release in the media expressed as a percentage (%) of positive control (LDH levels in media from a PCS where maximal death was induced by multiple freeze/thaws – normalized to media volume) where p values = 0.0044 and 0.0004 for IT-603 and IT-603+TGFβ1 respectively. Images are representative of n=3 independent slice experiments. Data are mean ± s.e.m. and representative of slices generated from 3 independent donors performed in duplicate. Scale bars equal 100 microns. P values were calculated using two-way ANOVA with Tukey post- hoc t-test (*P<0.05, **P<0.01 and ***P<0.001).