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. Author manuscript; available in PMC: 2020 Dec 14.
Published in final edited form as: Mech Ageing Dev. 2020 Jul 29;190:111322. doi: 10.1016/j.mad.2020.111322

Fig. 5.

Fig. 5

Presence of UVB-irradiated HDFs induces multinucleation and modulate melanogenesis of melanocytes in a co-culture system. (A) Schematic representation of the experimental setup for co-culture. (B) Analysis of cell surface area was performed in ImageJ. At least 30 cells from three independent experiments were analyzed. (C) Percentage of multinucleated cells was calculated for melanocytes cultivated in the presence of UVB-irradiated or control HDFs on D3. (D) Intracellular melanin content measurement was performed on D3 of co-culture. Bars represent mean values of three independent experiments ± SD. (E) Representative pictures of melanocytes stained by FM. Scale bar 50 μm. Insets display detail of cytoplasmic regions containing melanosomes (F) The FM stained melanocytes were separated into groups according to the number of melanosomes per cell. Results are presented as mean values ± SD of three independent replicates. * < 0.05; ** < 0.01; *** < 0.001 and n.s. no statistical significance.