Figure 4. Effect of environmental conditions on cells. Converged printing increases printing time of constructs, which is related to the volume and line spacing of the prints A). Converged printed constructs with a volume of 100 mm³ and a line spacing of 400 μm, resulted in 10 min of printing time per construct. Incubating the hydrogel after crosslinking decreased metabolic activity of the cells B). Metabolic activity was normalized against cast control sample without incubation time. * = significant difference from an incubation time of 0 min, p < 0.05.