Extended Data Table 2. Cryo-EM data collection, refinement and validation statistics.
| 3 Closed RBDs (EMD-11497, PDB 6ZWV) | 2 Closed + 1 Weak RBDs (EMD-11498) | ||
|---|---|---|---|
| Dala collection and processing | |||
| Magnification | 81,000 | 81,000 | |
| Voltage (kV) | 300 | 300 | |
| Electron exposure (e-/Å2) | 50 | 50 | |
| Defocus range (μm) | 1.0-3.0 | 1.0-3.0 | |
| Pixel size (Å) | 1,061 | 1,061 | |
| Movies (no.) | 7,982 | 7,982 | |
| Symmetry imposed | C3 | Cl | |
| Initial particle images (no.) | 450,473 | 450,473 | |
| Final particle images (no.) | 29,183 | 25,976 | |
| Map resolution (Å) | 3.5 | 4.1 | |
| FSC threshold | 0.143 | 0.143 | |
| Map resolution range (Å) | 3.35-27.16 | 3.67-15.27 | |
| Refinement | |||
| Initial model used | PDB 6ZP0 | ||
| Model resolution (Å) | 3.5 | ||
| FSC threshold | 0.5 | ||
| Map sharpening B factor (Å2) | -50 | ||
| Model composition | |||
| Non-hydrogen atoms | 24036 | ||
| Protein residues | 2955 | ||
| Ligands | 63 | ||
| B factors (Å2) | |||
| Protein | 130.57 | ||
| Ligand | 142.74 | ||
| R.m.s. deviations | |||
| Bond lengths (Å) | 0.009 | ||
| Bond angles (°) | 1.346 | ||
| Validation | |||
| MolProbity score | 1.62 | ||
| Clashscore | 2.97 | ||
| Poor rotamers (%) | 1.74 | ||
| Ramachandran plot | |||
| Favored (%) | 94.97 | ||
| Allowed (%) | 5.03 | ||
| Disallowed (%) | 0.00 |