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. Author manuscript; available in PMC: 2020 Dec 21.
Published in final edited form as: Nat Struct Mol Biol. 2020 Apr 27;27(5):450–460. doi: 10.1038/s41594-020-0418-4

Fig. 6. PCNA monoubiquitination promotes lagging-strand TLS.

Fig. 6

(a) Standard replication reaction time course performed with wild-type PCNA in the absence or presence of Uba1 on the lagging-strand CPD template. Reactions contained Pol η, Pol δ, ubiquitin, Rad6–Rad18, Fen1 and Ligase, in addition to standard replication proteins.

(b) Quantification of the percentage of bypass in the absence or presence of Uba1 as performed in (a). Data are plotted as the means of three independent experiments with error bars representing the SEM.

(c) Standard replication reaction time course as performed in (A) but with PCNAK164R.

(d) Quantification of the percentage of bypass in the absence or presence of Uba1 as performed in (c). Data are plotted as the means of three independent experiments with error bars representing the SEM.

Uncropped versions of Fig. 6 gels are available in Source Data Fig. 6.