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. Author manuscript; available in PMC: 2020 Dec 22.
Published in final edited form as: Nature. 2015 Jan 5;520(7545):114–8. doi: 10.1038/nature13993

Extended Data Figure 5. Analysis of DSB double-cutting at various genomic loci.

Extended Data Figure 5

a-b, Agarose-embedded genomic DNA isolated from the indicated timepoints and strains was fractionated by PFGE, transferred to nylon and hybridised with various probes: FRM2 (a); POL5, DOT5, CTR86, YCR061W (b). a, Detection of double-cut (left panel) and quantification (right panel). Major double-cut band corresponding to coincident DSBs at HIS4::LEU2 and leu2::hisG is indicated with a star. b, Detection of double-cuts on different chromosomes following PFGE in strains fully (dmc1Δ exo1Δ and sae2Δ) or partially (exo1Δ) defective for DSB repair (top panel). Asterisks: tel1Δ-specific double-cut signals. Diagram depicts possible double-cuts (bottom panels).