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. Author manuscript; available in PMC: 2020 Dec 30.
Published in final edited form as: Virology. 2006 Jul 26;353(1):17–26. doi: 10.1016/j.virol.2006.04.022

Fig. 3. Localization of the region of M6 RNA bound by NS2.

Fig. 3

(A) Schematic of the truncations in M6 plus-strand RNA used as competitors in EMSA. Three fragments, M6a (nt 1–868), M6b (nt 449–1352, M6c (868–1769) were used. (B) EMSA data. The identity of the M6 truncation used as competitor for binding of the full-length probe is shown above each panel. Lane 1 contains 1× 10−9 M 32P-labeled full-length M6 probe only; lane 2, 32P-labeled probe and NS2 and lanes 3–8 contain 32P-labeled probe and NS2 and unlabeled competitor in molar ratio of probe: competitor of 1:10, 1:100, 1:300, 1:600, 1:1000 and 1:10,000 respectively.