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. Author manuscript; available in PMC: 2021 Jan 4.
Published in final edited form as: Nat Chem. 2020 May 29;12(6):535–544. doi: 10.1038/s41557-020-0472-x

Figure 2. The MmPylRS/Mm PyltRNA pair is not orthogonal with respect to any ΔNPyltRNAs and some ΔNPylRSs.

Figure 2

a, GFPHis6 fluorescence generated by each PylRS with Mm PyltRNACUA resulting from GFP(150TAG)His6 in presence of BocK 1. Each bar represents the mean of four biological replicates ± s.d.. The individual data points are shown as dots. All numerical values are provided in Supplementary Table 3. b, GFPHis6 fluorescence generated by MmPylRS with each PyltRNACUA, amd GFP(150TAG)His6 in presence of BocK 1. Each bar represents the mean of three biological replicates ± s.d. for class B ΔNPyltRNACUAs or four biological replicates ± s.d. for class A ΔNPyltRNACUAs and Mm PyltRNACUA. The individual data points are shown as dots. c, Schematic of the functional interactions between class A and class B ΔNPylRS/ΔNPyltRNA pairs in relation to each other and MmPylRS/Mm PyltRNA. Black arrows indicate high activity, grey arrows indicate low activity and the dashed gray arrow indicates minimal activity corresponding to functional orthogonality.