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. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Nat Protoc. 2020 Nov 18;16(1):27–60. doi: 10.1038/s41596-020-00404-1
Component For 150 μL For 450 μL*
Water 133.65 μL 401 μL
10x RNase buffer 15 μL (final: 1x) 45 μL
1M DTT 0.75 μL (final: 5 mM) 2.25 μL
10 mg/mL RNase A 0.2 μL (final: ~100 U) 0.6 μL
RNase T1 0.5 μL (final: ~100 U) 0.5 μL
*

use if 3 SILAC samples were combined