| Component | Amount per reaction |
|---|---|
| SYBR Green PCR Master Mix | 5 μL (final: 1X) |
| Sample diluted 1:50-1:100 | 5 μL |
| 100 μM forward primer | 0.05 μL (final: 0.5 μM) |
| 100 μM reverse primer | 0.05 μL (final: 0.5 μM) |
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