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. Author manuscript; available in PMC: 2021 Jan 8.
Published in final edited form as: Nat Neurosci. 2020 Mar 16;23(4):500–509. doi: 10.1038/s41593-020-0602-1

Figure 5. Evidence that post-mitotic neuronal cues establish astrocyte layer identities.

Figure 5

A-D) Satb2 cKO mice show defects in upper layer neuron and astrocyte identity.

A) Images showing aberrant upper neuronal layers in the Satb2 cKO barrel cortex at P14.

B) Single astrocyte maps of layer astrocyte marker gene expression.

C-D) Quantification of cortical depth binned layer neuronal (C) and astrocyte (D) marker expression in cKO vs control.

E-H) Reeler mice show inversion of neuronal and astrocyte layers.

E) Images showing neuronal layer inversion in the Reln-/- barrel cortex at P14.

F) Single astrocyte maps of layer astrocyte marker gene expression.

G-H) Quantification of cortical depth binned layer neuronal (G) and astrocyte (H) marker expression in cKO vs control.

I) Diagrams depicting layer neuron and astrocyte changes in Satb2 cKO and Reln-/- mice.

n= 3 mice per genotype independently assayed, 5 tissue sections from each replicate imaged.

Scalebars: 100 µm. All data represent mean ± s.d. : Two-tailed Student’s t-tests were used with *P < 0.05,**P < 0.01, ***P < 0.001.