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. Author manuscript; available in PMC: 2021 Jan 8.
Published in final edited form as: Nat Neurosci. 2020 Mar 16;23(4):500–509. doi: 10.1038/s41593-020-0602-1

Figure 6. Astrocyte arealization across the cortex.

Figure 6

A-C) Single cell mapping of astrocyte gene expression across cortical areas. Maps show the (A) P14 cortical areas used in the analysis, (B) single astrocyte expression of Scel and (C) Chrdl1 across the dorsoventral and rostrocaudal extent of the cortex. Astrocytes are plotted as solid circles and colored quantitively for RNA spot counts per gene per cell.

Arrowheads indicate the restriction of Scel expression to sensory areas. Abbreviations as in Fig 1.

D) Astrocyte layers are distinct across cortical areas. Smoothened tile plots showing the quantification of neuronal Rorb expression and astrocyte expression of Scel, Chrdl and Il33 across the cortical areas and layers

E) Diagrams showing the divergent layer heterogeneity of astrocytes. Our study identifies superficial (sAS), mid (mAS) and deep (dAS) layer astrocyte subtypes through cortical gray matter in the postnatal cortex and confirms white matter like (wlAS) properties of L6 astrocytes.

F) 3D model showing astrocyte area and layer heterogeneity. Astrocyte layering is regionally specialized across the dorsoventral and rostrocaudal extent of the cortex. n = 1 mouse, 10 tissue sections independently imaged.