Extended Data Figure 3.

a, Immunofluorescence analysis of YAP1 (green), F-ACTIN (red), WWTR1 (magenta) and DAPI nuclear staining (blue) in a human morula stage embryo (n = 3). b, Immunofluorescence analysis of YAP1 (green), NANOG (red) and WWTR1 (magenta) and DAPI nuclear staining (blue) in a human blastocyst stage embryo (n = 3). Yellow arrowheads point to outer and TE cells co-expressing YAP1 and WWTR1. c, Immunofluorescence analysis of GATA3 (green), KRT18 (magenta) and HOECHST-33342 nuclear staining (blue) in a human blastocyst stage embryo (n = 3). d, Genome browser view of the ATAC-seq signal at the GRHL2 locus. High confidence peaks (FDR < 0.001) were used to identify transcription factor motifs. Representative binding motifs associated with the footprints are highlighted. The average expression of GRHL2 in high GATA3-high and GATA3-low expressing cells at the morula is shown and the TPM units indicated. Scale bars, as displayed in figures. e, Immunofluorescence analysis of GRHL2 (green), F-ACTIN (red), GATA3 (magenta) and DAPI nuclear staining (blue) in human morula stage embryos (n = 3). Yellow arrowheads point to outer cells co-expressing GRHL2 and GATA3, and cyan arrows point to inner cells expressing GRHL2. f, Immunofluorescence analysis of GRHL2 (green), OCT4 (red), GATA3 (magenta) and DAPI nuclear staining (blue) in human expanded and hatching blastocyst stage embryo (n = 3 each stage). Scale bars, as displayed in figures.