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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Nat Chem. 2020 Jul 13;12(9):852–859. doi: 10.1038/s41557-020-0504-6

Figure 1. Design of a square-shaped DNA origami structure and its assembly into 2x2 arrays and crystals.

Figure 1

a, Molecular model of the DNA nanostructure. A detailed AFM image shows the origami structure and its cavity. For orientation the outer edges of the structure are addressed with North, South, East and West. The zoomed-in image of the symmetric cavity shows the 12 staples available for modification, and their respective numbers. These staples are used to arrange the DNA aptamers in a controlled orientation and position. b, Scheme and AFM images of a 2D crystal generated from square-shaped DNA origami structures. Connecting origami edges in the way shown in the scheme results in larger and more homogeneous crystals than other connection modes. U-shaped barcodes are used to visualize the connection between monomers in the crystal. Green boxes in the scheme and small AFM image highlight a single structure as well as a line of structures within the crystalline assembly. The large AFM image indicates the size and regularity of the crystals achieved in this way c, Schemes and AFM images of the 2x2 arrays and their respective barcoding. The barcodes represent digital numbers that label uniquely addressable arrays.