Extended Data Figure 7. Re-LPS contact mapped onto LptDE.

(a) Average Re-LPS contacts mapped onto cartoon representations of LptDE. (b) Residues interacting with the three Re-LPS lipids referenced in Figure 2c (Re-LPS(1-3)) are shown in stick representation. (c) C-terminal strand of the βtaco is in contact with the bilayer (Supplementary Fig. 15c). (d) Ile186 and Phe187 are in transient contact with the Re-LPS lipids engaged with the lateral putative exit gate of the β-barrel. The open gate causes Re-LPS lipids (shown in yellow and pink) to be laterally pulled into the β-barrel sinking them into the inner leaflet of the and allowing the tails to interact with the C-terminal strand of the β-taco. This may suggest how Re-LPS gets laterally extruded into the OM during the translocation process.