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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Nat Immunol. 2020 Dec 21;22(2):140–153. doi: 10.1038/s41590-020-00824-x

Extended Data Fig. 5. SYK is required for GFP influx into phagosomes.

Extended Data Fig. 5

a, Western blot and confocal microscope images of SYK protein expression in HEK293T SYK KO cells, complemented with mouse mCherry::SYK or mCherry::SYK K396R kinase dead (KD). Bottom panel: mCherry expression of the same micrographs depicted as in Fig. 5c lower panel (scale bar 2 μm). b, Flow cytometry plots measuring phagocytosis of HEK293T WT and SYK KO cells incubated with zymosan-pHrodo-Red particles over 30 min timecourse. Data are representative of two independent experiments (n = 2).