Skip to main content
. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Nat Immunol. 2020 Dec 21;22(2):140–153. doi: 10.1038/s41590-020-00824-x

Abbreviations: FC (flow cytometry), CI (cell isolation)

Target Conjugate Clone Stock (mg/ml) Application Company
CD4 PE RM4-5 0.2 CI 1:200 BD Biosciences 553049
CD8a BV785 53-6.7 0.2 FC: 1:200 Biolegend 100750
CD11b PE M1/70 0.2 CI: 1:200 BD Biosciences 553311
CD11c PE HL3 0.2 CI: 1:200 BD Biosciences 553802
CD16/32 PE 2.4G2 0.2 CI: 1:200 BD Biosciences 553145
CD40 PE 3/23 0.2 FC: 1:200 BD Biosciences 553791
CD45.1 AF647 A20 0.5 FC: 1:200 Biolegend 110720
CD45.2 BV510 104 0.2 FC: 1:200 Biolegend 109838
CD44 FITC 1M7 0.5 FC: 1:200 BD Pharmingen 553133
CD45R (B220) PE RA3-6B2 0.2 CI: 1:200 BD Biosciences 553090
CD62L PE-Cy7 MEL-14 0.2 FC 1:200 Biolegend 104418
TCRβ BV421 104 0.2 FC 1:200 Biolegend 109230
CD107b (LAMP-2) FITC M3/84 0.5 FC 1:200 Biolegend 108504
CD107b (LAMP-2) AF647 M3/84 0.5 FC 1:200 BD Biosciences 564843
DNGR-1 PE 1F6 0.2 FC: 1:50 Biolegend custom
DNGR-1 APC 1F6 0.2 FC 1:50 Biolegend custom
H-2Kb (MHC I) APC AF6-88.5 0.2 FC 1:200 Biolegend 116518
H-2Kd (MHC I) FITC SF1-1.1 0.2 FC 1:200 BD Pharmingen 553565
I-A/I-E (MHC II) BV711 M5/11 4.15.2 0.2 FC 1:200 BD Biosciences 563414
Kb/OVA257 (SIINFEKL) tetramer PE - 2 FC 1:400 Baylor College of Medicine
Pro5® MHC pentamer H-2Kb SIINFEKL R-PE - - FC 1:100 ProImmune
Interferon-γ biotin XMG1.2 0.5 ELISA 1:1,000 BD Biosciences 554410
Interferon-γ PE XMG1.2 0.2 FC 1:100 Biolegend 505808
Ly6c/Ly6g (Gr-1) PE RB-8C5 0.2 CI 1:200 Biolegend 108408
XCR-1 PE ZET 0.2 CI 1:50 Biolegend 148204
alpha-Tubulin HRP 11H10 n/a WB: 1000 Cell Signaling 9099S