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. Author manuscript; available in PMC: 2021 Aug 1.
Published in final edited form as: Nat Immunol. 2020 Dec 21;22(2):140–153. doi: 10.1038/s41590-020-00824-x

Abbreviations: FC (flow cytometry), IF (immunofluorescence)

Target Conjugate Species Stock (mg/ml) Application Company
biotin alkaline phosphatase Extravidin from egg white avidin 1.5 ELISA 1:5,000 Sigma E2636
Goat IgG AF647 donkey 2 FC 1:200 Thermo Fisher A21447
Rabbit IgG AF555 polyclonal donkey 2 IF 1:200 Thermo Fisher A31572
Rabbit IgG AF647 polyclonal goat 2 FC 1:200 IF 1:200 Thermo Fisher A21245
Rabbit IgG BV421 polyclonal donkey 0.4 FC 1:200 Biolegend 406410
Rat IgG Cy5 polyclonal goat 2 IF 1:200 Thermo Fisher A10525
Rabbit IgG HRP Goat polyclonal 1 WB:1:2000 Jackson Immunoresearch 111035003