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. Author manuscript; available in PMC: 2021 Feb 2.
Published in final edited form as: Nat Cell Biol. 2020 May 11;22(6):621–629. doi: 10.1038/s41556-020-0515-y

Extended Data Fig. 1.

Extended Data Fig. 1

Dynamics of histone H3.1 and H3.3 during pollen development.

Expression of H3.1 (a-d) and H3.3 (e-f) isoforms during pollen development. Histone H3.1 is encoded by five isoforms: HTR1 (a), HTR2 (b), HTR3 (c), HTR9 and HTR13 (d). Histone H3.3 is encoded by three isoforms: HTR4, HTR5 (e) and HTR8 (f). Two pairs of genes (HTR4-HTR5 and HTR9-HTR13) are found in tandem at the same locus so a single reporter for each pair was used to monitor expression. Histone H3.1 (HTR2, HTR3 and HTR13) were detectable in the microspore and sperm precursor but this signal disappeared rapidly before sperm mitosis. No pollen expression was detected for HTR1. Histone H3.3 (HTR5 and HTR8) were detected throughout pollen development but had a much reduced (HTR5) or absent (HTR8) signal in sperm. Arrows indicate expression in the microspore or VN while arrowheads distinguish expression in the sperm lineage. The marker line analysis was repeated twice with independent inflorescences. Scale, 5 μm.