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. Author manuscript; available in PMC: 2021 Feb 2.
Published in final edited form as: Symbiosis. 2020 Jul 27;82(1):123–131. doi: 10.1007/s13199-020-00697-6

Fig. 3.

Fig. 3

Coculture experiments of Hortaea werneckii and Dunaliella spp. a. Alginate inclusion (delineated by dot line) on BBM 10 M NaCl used to attempt isolation and cogrowth of H. werneckii and D. atacamensis from Atacama environmental sample. Soil debris and fungal + algae clumps are visible. b, c. Alginate inclusion (delineated by dot line) on MEA 10%NaClto attempt cogrowth of H. werneckii EXF-6656 (black hyphae) and D. salina (arrow) as observed after 1 week (b) and after 1 month (c). Note how H. werneckii completely overgrew the inoculum in this last case. d-f. Alginate inclusions on MEA 5% NaCl to attempt cogrowth of H. werneckii EXF-2000 (black cells in D, yeast colony in E and F) and D. salina (arrow), 1 week (D), 3 weeks (E) and 5 weeks (F) after inoculation. Note how H. werneckii completely overgrew the inoculum in this last case, and even addition digested the alginate used. g, h. Dialysis membrane filled with MEA medium (arrow) and inoculated with H. werneckii, submersed in Dunaliella medium. Scale bars: A, B) 1 mm; C) 4 mm; D, F) 2 mm; E) 5 mm; G, H) 2,5 mm