Table 1. Putative iNPE-Binding Protein Candidates Identified by LC-MS/MS.
PlasmoDB Accession | Annotation | Protein Probability (%) | MW (kDa) | Unique Peptides | Sequence Coverage (%) | Experiment 1 | Experiment 2 |
---|---|---|---|---|---|---|---|
PF11_0091a | Transcription factor with AP2 domain(s), putative | 100 | 206.9 | 3 | 1.48 | + | + |
MAL13P1.237 | Nucleic acid binding protein Alba, putative | 100 | 42.2 | 7 | 25 | + | + |
PF08_0074 | DNA/RNA-binding protein Alba, putative | 100 | 27.2 | 6 | 29 | + | + |
MAL13P1.233 | Nucleic acid binding protein Alba, putative | 100 | 25.0 | 4 | 22 | + | + |
MAL8P1.40 | RNA binding protein, putative | 100 | 32.4 | 8 | 30 | - | + |
PFI1025w | RNA binding protein, putative | 100 | 56.6 | 3 | 11 | - | + |
PF10_0068 | RNA binding protein, putative | 100 | 29.5 | 4 | 29 | + | + |
PF14_0096 | RNA binding protein, putative | 100 | 59.3 | 3 | 7 | - | + |
PFI1435w | RNA binding protein, putative | 99.8 | 33.6 | 3 | 10 | + | + |
PFE0435c | DNA binding protein, putative | 99.8 | 33.8 | 3 | 13 | + | + |
PFL1170w | Polyadenylate-binding protein, putative | 100 | 97.2 | 14 | 20 | + | + |
PFI0235w | Replication protein A | 100 | 56.2 | 16 | 40 | + | + |
PFL2215wb | Pfactin-I | 100 | 41.9 | 8 | 52 | + | + |
Accession numbers (http://www.plasmodb.org/) of putative DNA/RNA binding proteins exclusively identified in the int-c1 elution.
Pfactin-I has been validated by ChIP as a member of the iNPE-binding protein complex.