Fig. 3.

Microscope slide setup for FISH analysis. The Teflon-coated surface delimitates and reduces the region to apply the parasite suspension and solutions or antibodies. The rubber in situ frame when covered with a coverslip (also in plastic) creates a small hybridization chamber that allows a reduction on the amount of probe required for each assay. This system also prevents against evaporation during the overnight hybridization. The frames are adhesive on both sides, and can be applied and removed easily from the microscope slide using forceps. We usually use microscope slides with three wells (and three frames), thus allowing for testing three different conditions per slide.