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Red Fluorescence intensity histograms for each gel layer analysed every 2 hours. A) Histogram correspondent to inactivated nCol-F-Rh (nCol-A). B) Histogram correspondent to nCol-F-Rh preincubated at pH 7.2 (nCol-B). C) Histogram correspondent to nCol-F-Rh preincubated at pH 5.5 (nCol-C).
