Skip to main content
. Author manuscript; available in PMC: 2021 Feb 9.
Published in final edited form as: Nature. 2020 Jul 29;586(7827):133–138. doi: 10.1038/s41586-020-2541-0

Extended Data Fig. 5. Suppression of WNT target gene expression by mutant p53 is mediated by preventing the association of TCF4 with WNT promoters.

Extended Data Fig. 5

a, Immunoblot of mouse jejunal enterocytes. PP2Ac, loading control. For gel source data, see Supplementary Fig. 1. b, Immunoblot of cytoplasmic and nuclear extracts from mouse jejunal enterocytes. GAPDH and fibrillarin, cytoplasmic and nuclear markers, respectively. For gel source data, see Supplementary Fig. 1. c, d, TCF4 (c) and H3K4me3 (d) ChIP of WNT target promoters in mouse jejunal enterocytes. Mean enrichment ± s.e.m. (n, number of mice), one-way ANOVA with Tukey’s test. e, f, TCF4 (e) and H3K4me3 (f) ChIP of WNT target promoters in mouse ileal enterocytes. Mean enrichment ± s.e.m. (n, number of mice), one-sided Student’s t-test. g, H3K4me3 ChIP of WNT target promoters in crypt and villus fractions of mouse jejunum. Mean enrichment ± s.e.m. (n, number of mice), one-sided Student’s t-test. Representative data from three independent experiments (a, b).