| pKLV2-EF1a-Cas9Bsd-W |
Cas9 expression vector |
Addgene #68343 RRID:Addgene_68343 |
| pKLV2-U6gRNA5(BbsI)- ccdb-PGKpuro2ABFP-w |
gRNA vector, modified to contain ccdB gene between Bbs1 sites for gRNA subcloning. |
Gene Editing, Cellular operations, Wellcome Sanger Institute |
| psPAX2 |
VSV-G envelope expressing plasmid. |
Addgene #12260 RRID:Addgene_12260 |
| pMD2.G |
2nd generation lentiviral packaging plasmid. |
Addgene #12259 RRID:Addgene_ 12259 |
| pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W |
Cas9 activity reporter plasmid. |
Addgene #67980 RRID:Addgene_ 67980 |
| pKLV2-U6gRNA5(Empty)-PGKGFP2ABFP-W |
Cas9 activity reporter plasmid (control). |
Addgene #67983 RRID:Addgene_ 67983 |
