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. Author manuscript; available in PMC: 2021 May 11.
Published in final edited form as: Nature. 2020 Nov 11;588(7839):648–652. doi: 10.1038/s41586-020-2894-4

Extended Data Figure 8. L2/3 neurons receive presynaptic excitation preferentially tuned to their orientation preference.

Extended Data Figure 8

(a) Average tuning across the postsynaptic neurons responding to drifting gratings (N=16, mean ± s.e.m.), after alignment of their preferred stimulus direction to 0 deg. (b) Distribution of preferred direction relative to the postsynaptic preferred direction, for excitatory presynaptic ensembles across all layers (N=15, median ± m.a.d.). (c) Same as b, for excitatory presynaptic ensembles within L2/3 (top) and within L4 (bottom). (d) Same as b, for inhibitory presynaptic ensembles. (e) Average orientation tuning across the postsynaptic neurons responding to drifting gratings (N=16, mean ± s.e.m.), after alignment of their preferred stimulus orientation to 0 deg. (f) Average distribution of preferred orientation relative to the postsynaptic preferred orientation, for excitatory presynaptic neurons pooled across all layers (N=15, median ± m.a.d.). Presynaptic ensembles tuning for orientation (pkw = 5*10-8, two-sided one-way Kruskal-Wallis test) was significantly stronger than expected from random samples of the surrounding population (grey, median±m.a.d.). (g) Same as f, for presynaptic ensembles within L2/3 (top, N = 15, pkw = 10-7) and within L4 (bottom, N= 13, pkw = 2*10-5). (h) Same as f, for inhibitory presynaptic ensembles. (i) The tuning of the distributions of preferred orientation of excitatory presynaptic ensembles across layers plotted against the preferred orientation of their postsynaptic neuron. The co-tuning for orientation (r = 0.75, circular correlation, pr = 4*10-3, Z-test; pv = 9*10-8 circular V-test) was stronger than expected from random samples of the local population (pr_rand < 10-4 for circular correlation, pv_rand < 10-4 for V statistic). Upwards triangles represent experiments in CaMK2-GCaMP6 mice (N=11); downward triangles indicate experiments in GAD2-NLS-mCherry mice (N=4). (j) Same as i for excitatory presynaptic ensembles within L2/3 (top, r =0.92, pr = 5*10-3, pv = 10-5, pr_rand = 2*10-4, pv_rand < 10-4, N = 15) and within L4 (bottom, r = 0.76, pr = 2*10-2, pv=3*10-3, pr_rand = 0.18, pv_rand = 3*10-3, N=13). (k) Same as i for inhibitory presynaptic ensembles. Inhibitory presynaptic ensembles were weakly biased to the orientation preference of the postsynaptic neuron (pv = 0.05, circular V-test, N=4).