Extended data figure 7. RP deficiency alters the activity of an autophagy reporter.

(a) Cartoon showing progression through autophagy as monitored by the GFP-mCherry-Atg8a reporter. Yellow indicates the simultaneous presence of GFP and Cherry in the phagophore and autophagosome. Autolysosomes only retain the red colour because of the drop in pH, which quenches GFP fluorescence. (b, c) Fluorescence from GFP-mCherry-Atg8a, expressed with tubulin-GAL4 in wild type or RPS23^R67K/+. Single fluorescence channels are also shown in grey. Scale bars represent 50 μm. Genotypes for each figure panel are available in Supplementary Table 1. Experiments were repeated independently three times with similar results.