Extended data figure 9. Validating the effect of GADD34 overexpression.

(a) P-eIF2α immunoreactivity in a wild type imaginal disc. (b) This is reduced by GADD34 overexpression (GADD34^OE) driven by nubbin-GAL4. (c) P-eIF2α immunoreactivity is similarly decreased in RPS23^R67K/+ larvae overexpressing GADD34. (d) Schematic representation of the domain where GADD34 was overexpressed. (e,f) GADD34 overexpression causes a mild but significant increase in wing size in otherwise wild type flies but not in RPS23^R67K heterozygotes. Note that the wing of RPS23^R67K heterozygotes is smaller than that of wildtype. (g-i) GADD34 overexpression exacerbates the formation of HTT25Q punctae in RPS23^R67K heterozygotes. Statistics: error bars denote standard deviation. In f, n = 12 adult wings for each genotype. In i, from left to right, n = 10 and 8 discs. A two-tailed unpaired t-test was carried out. P-values in f, from top to bottom: 2.48E-01, 1.31E-07 and 7.09E-06. P-value in i, 9.65E-04. Scale bars represent 50 μm. Genotypes for each figure panel are available in Supplementary Table 1. Source data is available for this figure.