Extended data figure 10. Effect of proteasome inhibition on the rate of apoptosis in RP-deficient tissues.

(a) Extent of apoptosis (coverage of Dcp1 immuno-reactivity) in the pouch of discs of genotypes indicated. Rpt6 ^RNAi denotes rotund-gal4-driven expression of a Rpt6^RNAi transgene.. This particular Rpt6^RNAi transgene had only a minor effect on apoptosis in wildtype tissue. Expression of this RNAi transgene did not enhance the rate of apoptosis in RPS23^R67K heterozygotes. (b) The effect of stronger proteasome knockdown (with Rpn2^RNAi) on the rate of apoptosis in RP-deficient tissue could not be assessed because it triggered extensive apoptosis in otherwise wild type imaginal discs. Statistics: error bars denote standard deviation. n = 9 discs for each genotype. A two-tailed unpaired t-test was carried out. P>0.05, no significant difference was seen. Genotypes for each figure panel are available in Supplementary Table 1. Source data is available for this figure.