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. Author manuscript; available in PMC: 2021 Jul 25.
Published in final edited form as: Nat Cell Biol. 2021 Jan 25;23(2):127–135. doi: 10.1038/s41556-020-00626-1

Figure 4. The ISR and autophagy limit tissue damage caused by RP-deficiency.

Figure 4

(a) Relatively low number of apoptotic figures (Dcp1, green) in imaginal discs that overexpress GADD34 throughout the wing pouch (with nubbin-GAL4). (b,c) The rate of apoptosis in heterozygous for RPS23R67K is markedly enhanced upon GADD34 overexpression. (d) Quantification of apoptosis coverage (as in Fig.1d) in the three genotypes illustrated in panels a-c. (e) Relatively low number of apoptotic figures (Dcp1, green) in imaginal discs expressing an Atg1RNAi transgene throughout the wing pouch (with rotund-GAL4). (f,g) The rate of apoptosis in heterozygous for RPS23R67K is markedly enhanced upon Atg1RNAi expression. (h) Quantification of apoptosis coverage in the three genotypes of panels e-g. Statistics: in panel d, from left to right, n = 10, 7 and 10 discs. In panel h, from left to right, n = 21, 22 and 25 discs. All error bars denote standard deviation. A two-tailed unpaired t-test was carried out. P-values, from left to right in d: ***P = 9.43E-07, ***P = 5.36E-07 and in h, ***P = 2.52E-13, ***P = 1.35E-13. Scale bars represent 50 μm. Genotypes for each figure panel are available in Supplementary Table 1. Source data is available for this figure.