Extended data figure 3. Manipulation of Rheb activity affects OPP incorporation in wild type and RPS23^R67K/+ imaginal discs.

(a-d) Wing imaginal discs (wild type and RPS23^R67K/+, as indicated) overexpressing Rheb or Rheb^RNAi in the anterior compartment (left hand side of the disc). The discs were explanted and incubated for a 15 min in 1 μM OPP before staining for puromycilated peptides (grey scale). Rheb overexpression stimulated OPP incorporation in both genotypes, while Rheb^RNAi had the opposite effect. Scale bars represent 50 μm. Genotypes for each figure panel are available in Supplementary Table 1. Experiments were repeated independently three times with similar results.