Extended data figure 4. RPS23^R67K/+ imaginal discs develop tumours upon inhibition of apoptosis.

(a-b) Wing disc from a RPS23^R67K/+ larva expressing P35, a baculovirus-derived inhibitor of effector caspases in the pouch (under the control of rotund-GAL4). Note the overgrowth characterised by epithelial folds and ectopic Wingless expression (green), shown in grey scale at higher magnification in b. Formation of these tumours shows that RP-deficient cells are not inherently incapable of growth. Tumour formation may be relevant to the increase cancer risk associated with human ribosomopathies as well as to the observation that ribosomal protein genes are frequently deleted in human cancers, often in concert with the loss of TP53³. Scale bars represent 50 μm. Genotypes for each figure panel are available in Supplementary Table 1. Experiments were repeated independently three times with similar results.