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. Author manuscript; available in PMC: 2021 Feb 15.
Published in final edited form as: Nat Neurosci. 2020 Dec 21;24(1):47–60. doi: 10.1038/s41593-020-00757-6

Extended Data Fig. 3. Remyelination efficiency in conditional mutants.

Extended Data Fig. 3

(a) Expression of cholesterol synthesis genes in isolated astrocytes from EAE lesions. Bars represent the means with individual data points (n=4 animals) normalized to isolated cells from controls (n=4 animals, set to 1). Asterisks mark significant changes (Student’s t-test, two-sided).

(b) (Upper panel) Generation of MBP conditional mutants (MBPcKO) and (Lower panel) expression of cholesterol synthesis genes in isolated astrocyte 12 month post tamoxifen administration. Bars represent the means with individual data points (n=4 animals) normalized to isolated cells from control (n=4 animals, set to 1). Asterisks mark significant changes (Student’s t-test, two-sided).

(c) Tamoxifen protocol to induced recombination during cuprizone paradigm. Tamoxifen was given either before cuprizone application to target SQS in astrocytes, microglia, OPCs and endothelial cells or before remyelination to target oligodendrocytes.

(d) TDTO reporter expression during remyelination to evaluate cell targeting in cuprizone fed mice by co-labeling with CAII for oligodendrocytes, OLIG2 for oligodendrocyte linage cells, GFAP for astrocytes and Iba1 for microglia (Scale 50 μm).

(e) Elevated beam testing of cholesterol synthesis mutants during chronic remyelination (OLcKO n=8, OPCcKO n=7, AcKO n=5, McKO n=5, ECcKO n=7; OLcKO-Ctrl n=8, OPCcKO-Ctrl n=7, AcKO-Ctrl n=5, McKO-Ctrl n=5, ECcKO-Ctrl n=8). Bars represent the means with individual data points of three testing sessions. Asterisks mark significant changes (Student’s t-test, two-sided).

***p < 0.001, **p < 0.01.