Table 1.

Comparison of media composition. Lechner and LaVeck refers to the composition of LHC-9 medium published by those authors [21]. The difference between LHC-8 and LHC-9 is the presence of retinoic acid and epinephrine in the latter (Thermo Fisher Scientific). * In our experiments 100 nM retinoic acid was added to any already present for differentiation media; r = recombinant, h = human.

	BEGM (Lonza)		AECGM (PromoCell)		LHC-8 (Gibco)			PneumaCult-ALI
	Growth	Differentiation	Growth	Differentiation	Lechner & LaVeck (LHC-9)	Growth	Differentiation	Differentiation
FBS
BPE	?	?	0.4%	0.2%	0.5%	?	?
Epinephrine	?	?	0.5 μg /ml	0.25 μg/ml	0.5 μg /ml	2.7 μMa	1.35 μMa	?
Hydrocortisone	?	?	0.5 μg /ml	0.25 μg/ml	0.72 μg/ml	?	?	0.48 μg/ml
EGF	?rh	?rh	10 ng/mlrh	5 ng/mlrh	5 ng/ml	?	?	?
Transferrin	?h	?h	10 μg/mlrh	5 μg/mlrh	10 μg/ml	?	?	?
Insulin	?rh	?rh	5 μg/ml rh	2.5 μg/mlrh	5 μg/ml	?	?	?
Triiodothyronine	?	?	6.7 ng/ml	3.35 ng/ml	6.5 ng/ml	?	?	?
Retinoic Acid	?	? + 100 nMa	0.1 ng/ml	100.17 nMa	0.33 nM		100 nMa	?
Gentamycin	?	?			50 μg/ml			?
Amphotericin B	?	?				?	?	?
L-glutamine	?	?	6mM	5 mM	?	?	?	?
Phosphoethanolamine	?	?	?	?	0.5 μM	?	?	?
Ethanolamine	?	?	?	?	0.5 μM	?	?	?
Heparin						?	?	4 μg /ml
Human Serum Albumin			?	?		?	?	?

Not present

Present

Unknown