Extended Data Fig. 3. Single-particle cryo-EM analysis of FluPol_C-huANP32A and FluPol_C-chANP32A complexes.

a, e, Representative micrograph of FluPol_C-huANP32A (a) and FluPol_C-chANP32A (e) embedded in vitreous ice. Scale bar, 200 Å. b, f, Representative 2D class averages of FluPol_C-huANP32A (b) and FluPol_C-chANP32A (f). c, d, Data analysis for FluPol_C-huANP32A Subclass1 (c) and Subclass2 (d). 3D reconstruction locally filtered and coloured according to RELION local resolution (left panel). FSC curve indicating overall map resolution and model-to-map FSC (middle panel). Curves are shown for phase randomisation, unmasked, masked and phase-randomisation-corrected masked maps. Angular distribution of particle projections with the cryo-EM map shown in grey (right panel). g-j, Data analysis for FluPol_C-chANP32A Subclass1 (g), Subclass2 (h), Subclass3 (i) and Subclass4 (j). 3D reconstruction locally filtered and coloured according to RELION local resolution (top panel). FSC curve indicating overall map resolution and the model-to-map FSC (middle panel). Curves are shown for phase randomisation, unmasked, masked and phase-randomisation-corrected masked maps. Angular distribution of particle projections with the cryo-EM map shown in grey (bottom panel).