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. Author manuscript; available in PMC: 2021 Feb 17.
Published in final edited form as: Nature. 2020 Jul 29;587(7835):657–662. doi: 10.1038/s41586-020-2601-5

Fig. 1. DeISGylating and deubiquitylating activities of SCoV-PLpro and SCoV2-PLpro.

Fig. 1

a, SCoV-PLpro (left) or SCoV2-PLpro (right) were incubated with indicated Prg probes. Experiments were repeated three times independently with similar results. b, Catalytic efficiency (k cat/K m) of SCoV2-PLpro and SCoV-PLpro cleavage of K48-Ub2-AMC or ISG15-AMC. c, Dissociation constant (K d) of SCoV2-PLpro and SCoV-PLpro. Data in c, d, are mean ± s.d. or mean ± s.e.m. (n = 3 independent experiments). *P < 0.05, **P < 0.01, ****P < 0.0001; two-tailed paired t-test. d, Schematic representation of substrate specificity of SCoV2-PLpro (red) and SCoV-PLpro (blue). The preferred substrate is shown on the left.