Extended Data Table 3. Binding kinetics of PLpro to K48-Ub2 or ISG15.
| kon ± S.E.Ma | koff S.E.Ma | kd ± S.E.Ma | R2b | ||
|---|---|---|---|---|---|
| (102M-1s-1) | (10-1 s-1) | (μM) | |||
| CoV2 | Triazole linked K48-Ub2 | 158 ±8.00 | 4.30 ±0.11 | 27.28 ±1.55 | 0.99 |
| PLpro | HumanISG15 | 1530 ±52.4 | 2.15 ±0.06 | 1.41 ±0.06 | 0.99 |
| MouseISG15 | 122 ±4.48 | 0.20 ± 0.02 | 1.64 ±0.17 | 0.97 | |
| SARS PLpro | Triazole linked K48-Ub2 | 820 ±32.1 | 0.81 ±0.02 | 0.99 ± 0.04 | 0.98 |
| HumanISG15 | 88.8 ±14.5 | 0.76 ± 0.06 | 8.60 ±1.56 | 0.98 | |
| MouseISG15 | 24.7 ± 0.75 | 0.23 ± 0.01 | 9.34 ±0.41 | 0.98 |
Binding kinetic parameters for SCoV-PLpro, SCoV2-PLpro to triazole-linked K48-Ub2, humanISG15 or mouseISG15 are presented. Values are presented as mean ± s.e.m. (n = 3, independent experiments).
a
s.e.m.
b
R 2, goodness of the curve fit between experimental data and mathematical 1:1 binding curve