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. Author manuscript; available in PMC: 2021 Jul 11.
Published in final edited form as: Nat Microbiol. 2021 Jan 11;6(2):221–233. doi: 10.1038/s41564-020-00823-6

Extended Data Fig. 1. Purification of the GldLM’ and PorLM complexes.

Extended Data Fig. 1

Coomassie Blue-stained SDS-PAGE gels and size-exclusion column traces of complexes used for cryoEM structure determination. In each case the size exclusion column fractions that were pooled to make the cryoEM grids are denoted by an arrow and correspond to the fractions analysed on the gels. A280 is the absorption at 280nm in arbitrary units.

a, GldLM225(produced from pRHJ007). b, GldLM232 (produced from pRHJ008). c, PorLM (produced from pRHJ006).